ZIM3 and colorectal adenocarcinoma: Zim3-dCas9 also conferred strong and homogeneous knockdown in HepG2 (hepatocellular carcinoma), HT29 (colorectal adenocarcinoma), and HuTu-80 (duodenal adenocarcinoma) cells (Figure 4B, Figure 4—figure supplement 1) without negative impacts on cell proliferation (Figure 4—figure supplement 2).