ZIM3 and T-cell acute lymphoblastic leukemia: In both RPE1 (retinal pigment epithelium) and Jurkat (acute T-cell leukemia) cells, cells expressing Zim3-dCas9 had stronger knockdown than previously reported cell lines expressing dCas9-Kox1 (Figure 4A; Horlbeck et al., 2018; Jost et al., 2017).