To explore the effect of Wee1 in CML pathogenesis, we found that Wee1 knockdown was associated with reduced CML cell growth (Fig. 2a–c), as well as smaller (Fig. 2d) and fewer (Fig. 2e) cell colonies formation, and induced cell cycle arrest shown by PI staining (Fig. 2f). Here, WEE1 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.