The results of wound healing assay in HegG2, SMMC 7721 cells revealed that after 24 hours and 48 hours, the wound width or distance in SALL4 mimic group was significantly smaller and closer than that in SALL4 mimic-NC group, indicating that the migrated distance of tumor cells to the center of the wound increased significantly, indicating the SALL4 promoted the migration and invasion’ abilities in HCC cells. Here, SALL4 is linked to hepatocellular carcinoma.