IL10 and gastroenteritis: Further, we investigated the difference between the colonic DNA methylation of GF and SPF mice in a different genetic background, Interleukin 10 knockout (IL10KO), because IL10 is an anti-inflammatory cytokine and a previous study showed mice lacking the IL10 gene (IL10−/−, IL10KO) develop spontaneous intestinal inflammation in the presence of microbiota [29].