To further provide evidence that HzMUC1-MMAE induces apoptosis of pancreatic cancer cells, cells were treated the human IgG-MMAE (10, 20, 40 nM) or HzMUC1-MMAE (10, 20, 40 nM) for 72 h and then stained with propidium iodide (cell death marker)and annexin V (early apoptosis marker) (Fig. 4). Here, ANXA5 is linked to pancreatic neoplasm.