To understand the effect of ADs, methylation and their interaction on the transcriptional activity of BDNF exon IV, we cloned BDNF exon IV promoter region in a CpG-free luciferase vector [26] and transfected it into a neuroblastoma cell line SH-SY5Y and treated them with ADs 24 h after transfection for 48 h. This evidence concerns the gene BDNF and neuroblastoma.