SMAD3 and melanoma: To assess whether pErk1/2 hyperactivation is required for the basal migration priming of high SMAD3 fibroblasts, we analysed basal Transwell migration in the presence of 100 nM Trametinib, a clinically approved inhibitor of MEK1/2 MAP kinases in NSCLC and melanoma that acts right upstream of Erk1/2 [46].