To validate the enrichment of the EMT pathway in our KWC model, qRT-PCR for Ck-19, fibronectin, slug, Runx2, and Angptl4 of independent aged-matched RNA (2 months) of WT (n = 3), KC (n = 3), and KWC mice (n = 3) revealed an upregulation of these markers in KWC mice compared to KC mice (Fig. S3A–E). Here, ANGPTL4 is linked to keratoconus.