Wwoxfloxed mice (Wwoxf/+ or Wwoxf/f) [18] were bred with KC mice to generate KWC mice (Wwox(f/f or +/f);Kras+/LSL-G12D; Ptf1a-CreER; Rosa26-LSL-tdTomato) and WC mice (Wwox(f/f)/(+/f); Ptf1a-CreER; Rosa26-LSL- tdTomato) (Fig. 1A). This evidence concerns the gene PTF1A and keratoconus.