The mouse astrocytoma delayed brain tumor (DBT) cell line, which has been widely used in the MHV field to study the replication and propagation of different strains of the virus, was chosen as an immortalized cell line to further examine the effects of astrocyte MHV-A59 infection on ERp29 and Cx43. Here, GJA1 is linked to astrocytoma (excluding glioblastoma).