Indications of LPS-induced lung injury, including pulmonary histopathology, protein levels in BALF, the wet/dry ratio, total cell counts and neutrophil counts in BALF, and the levels of the proinflammatory cytokines TNF-α, IL-1β, and IL-6, demonstrated that our murine model of ARDS represents a powerful experimental tool to further investigate the pathologic processes of LPS-induced ARDS. This evidence concerns the gene TNF and acute respiratory distress syndrome.