Based on the KEGG pathway analysis, the differentially expressed FRGs were mainly enriched in the HIF-1 signaling pathway, ferroptosis, microRNA in cancer, the PPAR signaling pathway, bladder cancer, autophagy, programmed death ligand 1 (PD-L1) expression, and the PD-1 checkpoint pathway (Fig. 1C). The gene discussed is CD274; the disease is urinary bladder cancer.