Upstream regulator analysis revealed that LPS-like response (z scores were 3.30, 3.41, and 4.87, for ED, DBPCFC and MOUSE study groups, respectively), TNF (z scores were 2.58, 2.16, and 4.01, for ED, DBPCFC and MOUSE study groups, respectively), IL-6, NF-κB (z scores were 1.15, 2.40, and 2.00, for ED, DBPCFC and MOUSE study groups, respectively), EGF, and platelet-derived growth factor (PDGF-BB; z scores were 3.60, 1.98, and 2.58, for ED, DBPCFC and MOUSE study groups, respectively)) were the most significantly upregulated pathways during anaphylaxis in all three study groups. This evidence concerns the gene TNF and anaphylaxis.