In summary, our results indicated that the ginsenoside Rk3 is effective to inhibit proliferation, migration, and invasion by targeting miR-3677-5p/CXCL12 axis in SHI-1 cells, it provided experimental evidence for the role of Rk3 on antimonocytic leukemia, but to elucidate the exact mechanism remains to be further explored. This evidence concerns the gene CXCL12 and leukemia.