The rescue experiments showed that the silencing of DARS-AS1 or NAT10 alleviated proliferative potential, migration capacities, and invasion abilities, while this inhibition effect of the silencing of DARS-AS1 or NAT10 on these cellular activities was rescued by the silencing of miR-330-3p, suggesting that DARS-AS1 acts as a ceRNA through sponging miR-330-3p to enhance the expression of NAT10 in gastric cancer cells. The gene discussed is NAT10; the disease is gastric cancer.