The clinical analysis using the χ2 test (Table 1) showed that patients with high TNM stage or lymph node metastasis have higher DARS-AS1 expression, suggesting that DARS-AS1 expression correlates with T category (P = 0.040), N category (P = 0.016), and TNM stage (P = 0.005). The gene discussed is DARS1; the disease is metastatic malignant neoplasm in the lymph nodes.