The DARS-AS1/miR-330-3p/NAT10 axis interaction is involved in many tumor cellular activities, which may be an important regulatory axis in gastric cancer, whereas the detailed mechanism of DARS-AS1 in gastric cancer is still not clear and the DARS-AS1/miR-330-3p/NAT10 signaling pathway to regulate gastric cancer progression will be a study emphasis in further research. The gene discussed is DARS1-AS1; the disease is gastric cancer.