The results demonstrated that the expressions of HMGA1, HMGA2, HMGB1, HMGB2, and HMGB3 were high in GC cell lines (AGS, HGC-27, MGC-803, BGC-823, MKN-45, and MKN-28) compared to the normal gastric epithelial cells (GES-1), consistent with previous predictions using TCGA cohort. This evidence concerns the gene HMGB3 and gastric cancer.