We also observed high correlation coefficients between MIOS and GFPT1, SLC25A12 and MAPK8, PYCR1 and SLC25A22, LDHA and CXCL8, and MIOS and MAPK8. To overcome the over-fitting effect caused by a large number of samples in pan-cancer analysis, we randomly removed one of the paired genes (MIOS, SLC25A12, PYCR1, and LDHA) and further analyzed the 21 glutamine metabolism-related genes. This evidence concerns the gene LDHA and cancer.