By comparison, there were no significant differences between the two groups in almost all clinical and pathological features, such as sex, ASA, BMI score, distance from the anus, tumor differentiation, presurgery CEA level, presurgery CA19-9 level, cT, cN, ypT, ypN, TME quality, CRM status and so on (all P>0.05), except that the age of LNY <12 group was older than that of LNY ≥12 group (P<0.001), and LNY <12 group tended to have more TRG 0 cases, while LNY ≥12 group tended to have more TRG 3 cases (P<0.060). The gene discussed is CEACAM5; the disease is neoplasm.