The mRNAs of other genes such as CREB1, FASN, LGALS4, PTGS1, SCAF11, TNFRSF10D, and USP5 were differentially downregulated in EVs from the three stages of PC patients compared to healthy subjects at an average of -20 to -90 fold (Figure 4-Group1). The gene discussed is LGALS4; the disease is pachyonychia congenita.