Taken together, it is demonstrated that as the sole shared high-abundance protein involving osteoblast differentiation from CMs of MDA-MB-231 and PC3 cells, RRBP1 depletion with shRRBP1 in cancer cells enhanced the osteoblastic phenotype expression and ameliorated the bone lesions induced by bone metastatic cancer cells. Here, RRBP1 is linked to cancer.