HRK and colorectal carcinoma: To explore the expression of HRK in CRC, we used Real-time RT-PCR and Western blotting in 8 CRC cell lines, an immortalized colon mucosa epithelial cell line (FHC) and paired CRC and adjacent noncancerous mucosa tissues.Compared with FHC, HRK mRNA expression was down regulated in CRC cell lines (Figure 2A).