Mass spectrometry experiment comparing conditioned medium (CM) of ALS astrocytes to that of healthy control revealed up-regulation of extracellular matrix proteins (collagen, lumican, olfactomedin-like protein 3 and protein-lysine 6 oxidase) and also downregulation of antioxidant proteins (SOD1, SOD2, glutathione synthetase) and motor neuron pro-survival factors (miR-494-3p), resulting in elevated stress and senescence in both astrocytes and motor neurons [87, 88]. The gene discussed is SOD1; the disease is amyotrophic lateral sclerosis.