In this study, we used RNA-seq, behavioral tests, western blot (WB), enzyme-linkedimmunosorbent assay, and co-immunoprecipitation (Co-IP) to comprehensively investigate therole of transcription factor RASD2 in 9-hour fasting on the improvement of depression-likebehavior induced by ovariectomy and whether this effect is regulated by DRD2. The gene discussed is DRD2; the disease is major depressive disorder.