CRH and Anxiety: Our findings were the following: (1) PACAP levels were increased in the CeA, but not the BNST, following CSDS; (2) reducing PAC1R levels in the CeA via a viral vector containing a short hairpin RNA significantly attenuated CSDS-included body weight loss; (3) CeA PAC1R knock-down abolished CSDS-induced heightened anxiety-like behavior; (4) CeA PAC1R knock-down prevented CSDS-induced local increase in CRF levels.