ALKBH5 and central nervous system cancer: To clarify whether ZDHHC3 regulates the protein stability of PD-L1 in glioma, cycloheximide-chase assays were conducted, and the results demonstrated that ALKBH5-KO and ZDHHC3-SH treatment accelerated the degradation of the PD-L1 when compared with their control groups (Fig. 5A, B).