We generated a p16 negative cell line of a squamous cell carcinoma of the penis, and from this parental cell line three chemo-resistant sublines were established: one subline adapted to 500 ng/ml 5-FU, one subline adapted to 2500 ng/ml CDDP and one subline adapted to both drugs. Here, CDKN2A is linked to squamous cell carcinoma of penis.