To validate that the hypermethylation of SCNN1B promoter directly contributes to transcriptional silence of SCNN1B, we treated a panel of 3 CRC cell lines with 5-Aza-2′-deoxycytidine (5-Aza), a DNA methyltransferase (DNMT) inhibitor. Here, DNMT1 is linked to colorectal carcinoma.