Because MALAT1, PTBP1, and PSF had been proposed to act as a functional module in HCC, we attempted to verify this hypothesis by determining whether the regulatory effects of MALAT1 on the malignant biological behavior of HepG2 and HCCLM3 cells are dependent on PTBP1 and PSF. The gene discussed is PTBP1; the disease is hepatocellular carcinoma.