With the induction of CDKN2A expression by sgiACTR5, immunoblotting revealed a drastic reduction of CDK6, phospho-S780 Rb (p-Rb), and E2F1 protein level in the ACTR5-dependent HCC cells (Fig. 3A; HepG2 and SNU475). This evidence concerns the gene ACTR5 and hepatocellular carcinoma.