ACTR5 and cancer: To examine the impact of ACTR5 depletion over diverse cell types, we CRISPR-targeted ACTR5 in two additional HCC cell lines SNU182 and SNU475 and compared it to the non-HCC cancer cell types, including acute myeloid leukemia (MV4-11 and MOLM13), breast cancer (MDA-231 and MCF7), and glioblastoma (U251 and U87) (Fig. 1E).