We noted that three of the four sgACTR5 in the DepMap screens indeed missed the CRISPR-sensitive regions (NCS < −1.0) of ACTR5, causing a false-negative assessment of this HCC essential gene within the consortium databases (a similar issue was also noted for IES6 in the DepMap database; fig. This evidence concerns the gene ACTR5 and hepatocellular carcinoma.