To identify genes directly regulated by ACTR5 in HCC, we captured genomic DNA associated with the Twin-Strep–tagged ACTR5 (ACTR5-TST) from HepG2 using the Strep-Tactin XT beads (29) for high-throughput sequencing (Fig. 2B) and then overlapped the ACTR5-bound targets (525 genes) with the E2F_Pathway genes (145 genes). The gene discussed is ACTR5; the disease is hepatocellular carcinoma.