Rapamycin treatment induced higher LC3II and lower P62 expression levels, which indicated that Rapa promoted the autophagic flux of diabetic hearts subjected to I/R (Figure 3A‐C).Rapamycin significantly decreased the areas of myocardial infarction and the serum cTnI levels compared with NS treatment (Figure 3D‐F). This evidence concerns the gene TRERF1 and myocardial infarction.