This study did not use multiple staining protocol because large lymphoma cells and lymphocytes have its own morphology characteristics so they can be easily distinguished.PD-1 showed positive staining on TILs in 79.3% of DLBCL cases and LAG-3 showed positive staining on TILs in 78.8% of DLBCL cases.Both TIM-3 and TIGIT were positive on TILs in DLBCL cases (62.7% and 69.5%, respectively), but were also expressed on tumor cells(44.8% and 45.4%, respectively). This evidence concerns the gene TIGIT and diffuse large B-cell lymphoma.