Here, within human and murine glioma and squamous cell carcinoma cells, remarkable upregulation of PD-L1 was observed, and PD-1/PD-L1 blockade was verified via a competition ELISA (unpublished data), which further confirms the connection between epigenetics and immune regulation and the promising combination therapy with PD-1/PD-L1 blockade and HDACi. Here, CD274 is linked to squamous cell carcinoma.