To assess the initiation of infection in C8-D1A cells, infectious center assays were performed after electroporation of full-length RNAs transcribed from the cDNAs of WT and nsP3 MD mutant viruses to eliminate potential differences in virus attachment and entry (Figure 2A) and after infection with these viruses at low (0.5) and high (5) MOIs (Figure 2B). The gene discussed is SH2D3C; the disease is infection.