Assetta et al. utilized a proximity ligation assay (PLA) to show transient interactions between JCPyV and 5-HT2A, 5-HT2B, and 5-HT2C receptor subtypes at 5 min post-infection (mpi) [68]; however, no interactions were observed at 0 and 15 mpi, and the nanoscale distribution of these 5-HT receptors during entry and infection has not been studied. This evidence concerns the gene HTR2A and infection.