In particular, i.l. or i.t. administration of free aPD-1 mAbs combined with PTX-loaded PPS micelles administered i.l. significantly expanded stem-like CD8+ T cells (Tcf-1+Tim-3-PD-1+ CD45+CD3+CD8+) systemically in triple-negative breast cancer (TNBC) E0771 tumor models after selective accumulation in TdLNs, leading to the control of distant and local tumors [5]. The gene discussed is CD8A; the disease is triple-negative breast carcinoma.