Solid lipid NPs (SLP) containing cationic phospholipids (152 nm) could not only physically load mRNA encoding OVA, tyrosinase-related protein 2 (TRP-2), or point-mutated version of glycoprotein 100 (gp100) that are melanoma self-antigens but also efficiently drain into the dLNs [84], ultimately resulting in improved antigen-specific (tetramer+) CD8+ T cell (CD45+CD3+CD8+) expansion and efficient tumor regression. The gene discussed is DCT; the disease is melanoma.