To determine whether the NRG-1 signaling is obligatory for the protection of AsVI against major depression, we transfected the PC12 cells with pcDNA3.1-NRG-1 in the CORT model cohort to overexpress NRG-1 while transfecting the cells with shNRG-1 in the CORT + AsVI cohort to inhibit the NRG-1 expression. Here, NRG1 is linked to major depressive disorder.