In our study, HFD promoted significantly differential expression of Cyp7a1, Lxra, Ppara, Atgl, Pgc1a, Leptin, Srebp1c, Il6, and Il1b, whereas CB-JT inhibited the differential expression of Lxra, Cyp7a1, Atgl, Srebp1c, Leptin, Il6, and Il1b. This result suggests that CB-JT prevented the development of obesity profiling partially by regulating cholesterol accumulation, energy metabolism, insulin sensitivity, and inflammatory processes. Here, PNPLA2 is linked to Obesity.