We also compared the virulomes of early- and advanced-stage CRC, and found that stages III and IV were more enriched with Fimbriae (FimB, FimE, fumH, FimI and FimG), transcriptional regulator (Ybta), outer-membrane A (Lps) and siderophores (aerobactin (iucb), enterobactin (fepC and fepG) and yersiniabactin (Ybtp, Irp1, and Irp2). This evidence concerns the gene ACO1 and colorectal carcinoma.