Our previous research proved that there were no obvious atherosclerotic plaques in the blood vessels of ApoE−/− mice at 8 w [22], but the OPLS-DA scatter plot in Figure 3A shows the distinctive grouping between C57 and ApoE−/− mice, indicating that the metabolic disorders have occurred in this stage without obvious atherosclerotic plaque. Here, APOE is linked to Other metabolic disease.