The spike-in concentrations were chosen to be physiologically relevant, the H1975-derived sEV concentration containing EGFR T790M (1 × 108 vesicles/mL plasma) was chosen to be about 100 times lower than the total reported 9.3 × 109 to 2.4 × 1010 vesicles/mL of healthy donor plasma since only a fraction of all sEVs in lung cancer patients are derived from tumor cells. Here, EGFR is linked to lung carcinoma.