EGFR and non-small cell lung carcinoma: In order to study the nucleic acid species encapsulated in sEVs that is most dominant and accessible as a template for mutation detection, the human NSCLC-derived NCI-H1975 (further called H1975) cell line containing the EGFR gene mutation c.2369C>T, p.T790M (hereafter called EGFR T790M) was used.