To investigate which compound exerts the best anti fibrotic activity, we compared the regulatory effects of ivermectin, abamectin, doramectin, selamectin, moxidectin, and eprinomectin on the activation of HSCs, and found that ivermectin had the most significant inhibitory effect on the expression of α-SMA in TGF-β1-treated CFSC cells, indicating that among these macrocyclic lactones, ivermectin should be the most effective against liver fibrosis (Figure S1). The gene discussed is ACTA1; the disease is Hepatic fibrosis.