SOD1 and amyotrophic lateral sclerosis: To test whether the nanobodies could induce degradation of ALS-linked SOD1 variants, HEK293T cells were co-transfected with either myc-tagged SOD1 WT (Figure 2A), SOD1 A4V (Figure 2B), or SOD1 G93A (Figure 2C), together with either nanobody, nanobody-PEST, or an empty control plasmid (i.e., the nanobody plasmid without the nanobody gene; Figure 2A–I).