ELANE and obesity due to melanocortin 4 receptor deficiency: Another study involved the use of spinning disk confocal intravital microscopy to visualize NETs by staining neutrophil elastase (NE) and Ly6G in obese mice, where NETs are impaired due to obesity because of the presence of dysfunctional platelets [81], which have been shown to play an important role in sepsis [82].