Increased renin mRNA expression in the clipped kidneys was associated with a significant increase in the circulating Ang II level in response to the development of 2K1C hypertension in WT mice at the end of experiment (Control: 35.8 ± 6.5 fmol/mL vs. 2K1C: 115.4 ± 17.8 fmol/mL, p < 0.01) (Figure 2E). This evidence concerns the gene REN and hypertensive disorder.