INS and metabolic dysfunction-associated steatotic liver disease: In another study, C57BL/6 male mice were fed an HFHF diet (consisting of 60% of kcal as fat and 35% fructose) for 16 weeks, which, apart from the aforementioned increases in hepatic free fatty acid content, serum insulin levels, insulin resistance and oxidative stress, further led to hepatic iron overload, a finding often observed in humans with NAFLD [48].