Administration of an HFD (21–23% fat, energy content 19.4–20.0 megajoule/kg, 43% energy as fat) to foz/foz mice leads to severe NASH progression with significant upregulation of ALT levels, hepatocyte ballooning, inflammation, and fibrosis, as well as further decreased serum adiponectin levels, increased cholesterol levels, and higher hepatic triglyceride levels [59,62]. This evidence concerns the gene GPT and metabolic dysfunction-associated steatohepatitis.