Analogous to these findings, the administration of a “diabetogenic” high-fat diet supplemented with cholesterol (35.5% carbohydrate, 36.6% fat, 0.15% cholesterol) leads to obesity, insulin resistance, hyperinsulinemia, dyslipidemia, increased hepatic triglycerides and ALT, exacerbated hepatic macrophage infiltration, apoptosis, and oxidative stress, as well as to micro- and macrovesicular steatosis, inflammatory cell foci, and fibrosis in the livers of the ldlr−/− mice [71]. This evidence concerns the gene GPT and obesity due to melanocortin 4 receptor deficiency.