Since NCS-1-KD had a pronounced effect on the generation of the first phase of the OGD-induced increase in [Ca2+]i in GABAergic neurons, which is a sign of hyperexcitation of the network, it was decided to test the role of NCS-1 in hyperexcitation of the neuronal network in a model of acute hyperammonemia. Here, NCS1 is linked to Hyperammonemia.