Our results have provided following novel findings: (1) cytokines have selective effects on the regulation of barrier properties of the colon: TNFα decreases barrier properties mainly in the distal part of the colon, while IL10 decreases the barrier properties only in the proximal one, (2) IL10 plays a prominent role in regulation of carcinogenesis and barrier function of the proximal part of the colon and may contribute to different incidences of CRC in the proximal and distal parts of the colon. Here, TNF is linked to colorectal carcinoma.