FASN expression was significantly negatively correlated with CD8+ T cells (BLCA, BRCA, CESC, HNSC, LGG, LUAD, LUSC, OV, SKCM, STAD), CD4+ T cells (BLCA, BRCA, ESCA, HNSC, PRAD, SARC, SKCM, STAD, TGCT, THCA), and DC cells (BLCA, HNSC, LGG, LIHC, LUAD, LUSC) infiltration in multiple cancer cell types using TIMER analysis, which is consistent with the ImmuCellAI results (Figure 4F). This evidence concerns the gene FASN and cancer.