PLIN1 and steatosis: When the presence of perilipin-positive microvesicular steatotic foci was correlated with the degree of steatosis as estimated by H&E or perilipin 2 immunostain, liver biopsies with perilipin-positive steatotic foci showed a significantly higher content of steatosis (11.67% and 8.06% in comparison to 6.85% and 6.14%; p < 0.05, Wilcoxon-text, Wilcoxon test effect sizes of 0.57 and 0.31; Figure 3c,d, Table 2).