PLIN1 and metabolic dysfunction-associated steatotic liver disease: In normal livers, irrespective of the species analysed, LDs and associated proteins were found mainly in the non-parenchymal HSCs (for perilipins 1 and 2 in bovine liver see Figure 1a,b);whereas, in human NAFLD, LDs were detected mainly in hepatocytes, and less were detected in non-parenchymal cells/HSCs (Figure 1d–f), although fluorescence microscopy of the human liver was in part hindered by large amounts of lipofuscin found in the hepatocytes of older patients.