Later, the particular phosphorylation sites of the NT5DC3 protein regulated by LF were screened by High-Performance Liquid Chromatography (HPLC) and validated by mutation experiments, further confirming that LF suppressed the malignant development from T2D to colon cancer by activating NT5DC3 phosphorylation modifications. Here, NT5DC3 is linked to malignant colon neoplasm.