Since the data showed that NT5DC3 was involved in the process of T2D development into colorectal tumors, and phosphorylation is considered an important protein modification to ensure normal physiological function [29,30], to investigate the mechanistic link between LF and NT5DC3 protein, the phosphorylation sites in NT5DC3 protein were screened by HPLC assay and confirmed by mutation assay, and the binding affinity between LF and NT5DC3 protein was measured by SPR. Here, NT5DC3 is linked to colorectal neoplasm.