Based on our results and considering the possibility of a well-defined function of overexpressed stemness markers in ESCC line KYSE-30 following enforced TWIST1 expression, it may be extrapolated that the ectopic expression of TWIST1 induced cells to undergo the EMT process for the acquisition of a CSC population and the self-renewal ability of stem-like cells. This evidence concerns the gene TWIST1 and esophageal squamous cell carcinoma.